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CO2 Incubators

CO2 incubators provide stable culture conditions for cell culture, tissue culture and microbiological research. We supply CO2 incubators from Thermo Scientific with HEPA filtration, copper interior and automatic CO2 control. From compact benchtop to large reach-in systems up to 820 litres.

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CO2 incubators for your laboratory

CO2 incubators provide stable culture conditions for cell culture, tissue culture and microbiological research. As a result, the unit precisely controls temperature, CO2 concentration and humidity to safeguard cell viability and sample integrity. Our CO2 incubators from Thermo Scientific use TC or IR sensors for accurate CO2 control, with optional O2 control for hypoxia research and tri-gas applications.

Which types of CO2 incubator do we supply?

Our range covers the full spectrum, from compact benchtop models to large reach-in systems for high-throughput laboratories.

Heracell VIOS CO2 incubator: the standard choice for modern cell culture applications. Available in capacities of 160 and 250 litres, with TC or IR sensor and optional copper interior for active antimicrobial protection. Moreover, the VIOS series offers fast recovery to optimal conditions after door openings.

Forma Steri-Cycle CO2 incubator: equipped with a built-in 140°C sterilisation cycle for maximum contamination prevention. Therefore, this model suits sensitive cell cultures, stem cell research and GMP environments where contamination is critical.

Heracell tri-gas CO2 incubator: a tri-gas configuration with CO2, O2 and N2 control for hypoxia research, stem cell culture under low oxygen and specific microbial applications. As a result, you can simulate physiological oxygen levels not achievable with a standard CO2 incubator.

Compact CO2 incubator: ideal for laboratories with limited floor space or for benchtop placement. Suitable for small research groups or as a secondary system alongside a larger model.

Large reach-in CO2 incubator (up to 820 litres): for high-throughput cell culture, monolayer production and biobanking. With multiple shelves, high volume and uniform air circulation, suitable for industrial scale-up.

Key technical features

HEPA filtration and ISO class 5: internal HEPA filtration with a closed air circulation system guarantees ISO class 5 air quality inside the culture chamber. As a result, contamination risks remain minimal.

Copper interior: active antimicrobial protection through copper. Moreover, the surface stays naturally free from bacteria, fungi and viruses without additional cleaning agents.

140°C sterilisation cycle: the Forma Steri-Cycle models offer a full 140°C sterilisation cycle. As a result, you reset the entire chamber to sterile conditions without disassembling the unit.

TC and IR sensors: TC sensors (thermal conductivity) are reliable and cost-efficient. IR sensors (infrared) are humidity-insensitive and recover faster after door openings. The choice depends on your protocol and lab humidity level.

Data logging and GMP compatibility: touchscreen interfaces, network connections and data logging functions support traceability for GMP and regulated environments. In addition, automatic calibration and alarm logging simplify audits.

Which CO2 incubator suits your application?

Routine cell culture: choose a Heracell VIOS 160 litre with TC sensor and HEPA filter. Suitable for most cell culture applications at an attractive price.

Stem cell research and sensitive cultures: choose the Forma Steri-Cycle with 140°C sterilisation cycle and copper interior. For hypoxia applications, choose a Heracell tri-gas.

High-throughput and biobanking: choose a large reach-in CO2 incubator from 400 to 820 litres. Stackable for maximum use of floor space.

Hypoxia and low oxygen research: choose a tri-gas configuration with CO2, O2 and N2 control. Essential for stem cell research under physiological conditions.

GMP and regulated environments: choose models with automatic calibration, data logging, network connection and extensive alarm functions.

Applications by sector

Medical diagnostics and clinical laboratories: cell and tissue culture, microbiological analyses and diagnostic cultures in medical diagnostic environments.

Pharmaceutical industry and biotech: high-throughput screening, monolayer production, stem cell research and development of cell therapy products in pharmaceutical processes.

Universities and research institutes: routine cell culture, molecular biology and microbiological research with flexible configurations for diverse protocols.

Biobanks: controlled long-term preservation of cell cultures, stem cell preparations and biological research material.

Food industry: microbiological analyses of food samples, fermentation research and shelf-life testing within the food industry.

Agriculture and horticulture: plant cell culture and development of biological crop protection agents within the agriculture and horticulture sector.

Service and maintenance on all CO2 incubators

Besides supplying and installing Thermo Scientific CO2 incubators, we provide periodic maintenance, HEPA filter replacement, sensor calibration, IQ/OQ qualification and NEN 3140 electrical safety inspection. In addition, our engineers perform contamination audits. Our certified service engineers work on CO2 incubators from all brands, including Eppendorf, Binder and Panasonic. For advice on the right CO2 incubator or a maintenance contract, please contact our specialists.

Frequently asked questions about CO2 incubators

What is a CO2 incubator?

A CO2 incubator is a laboratory device that provides stable culture conditions for cells, tissues and microorganisms. As a result, the unit regulates temperature (typically 37°C), CO2 concentration (typically 5%) and humidity (typically 95%) to simulate physiological conditions. A CO2 incubator is essential for cell culture, stem cell research and microbiological applications.

What is the difference between TC and IR sensors in a CO2 incubator?

A TC sensor (thermal conductivity) measures CO2 through conductivity and is cost-efficient and reliable. An IR sensor (infrared) measures CO2 through light absorption and is insensitive to humidity. Therefore, an IR sensor recovers faster after door openings and performs better in high-humidity environments. The choice depends on your protocol and throughput.

What is a tri-gas CO2 incubator?

A tri-gas CO2 incubator controls three gases simultaneously: CO2, O2 and N2. As a result, you can simulate physiological oxygen levels not achievable with a standard CO2 incubator. Tri-gas is essential for hypoxia research, stem cell culture under low oxygen and specific microbial applications.

Why a copper interior in a CO2 incubator?

A copper interior provides active antimicrobial protection. Moreover, the surface stays naturally free from bacteria, fungi and viruses without additional cleaning agents. As a result, a copper interior significantly reduces contamination risk, especially important for sensitive cell cultures and stem cell research.

What is the 140°C sterilisation cycle?

The 140°C sterilisation cycle is a built-in function of the Forma Steri-Cycle CO2 incubator. As a result, the incubator heats the entire chamber to 140°C for a controlled period. This kills all microorganisms in the chamber without disassembling the unit. This is ideally suited for GMP environments and sensitive cultures.

Which CO2 incubator brands does Poly Temp supply?

We are the official dealer of Thermo Scientific for the complete CO2 incubator range, including Heracell VIOS, Forma Steri-Cycle and Heracell tri-gas. Moreover, we provide maintenance and service on CO2 incubators from all other brands, including Eppendorf, Binder and Panasonic.

How often should a CO2 incubator be serviced?

Annual preventive maintenance is the standard for CO2 incubators. This includes HEPA filter replacement, sensor calibration (CO2 and temperature), inspection of the humidification pan and NEN 3140 electrical inspection. For intensive use or GMP environments, semi-annual maintenance is recommended. Our engineers also perform contamination audits.

How often should I replace the HEPA filter?

A HEPA filter in a CO2 incubator typically lasts 2 to 3 years under normal use. During annual maintenance we measure filter resistance. As a result, we know precisely when replacement is required. For intensive use or in dust-rich environments, replacement may be needed sooner.

Can Poly Temp also service a CO2 incubator from another brand?

Yes. Our certified service engineers perform maintenance, sensor calibration and HEPA filter replacement on CO2 incubators from all brands. This includes Eppendorf, Binder and Panasonic. Contact us for a tailored maintenance contract.

Which CO2 incubator capacity do I need?

For small research groups, a compact 50 to 90 litre benchtop suffices. Routine cell culture requires 160 to 250 litres (Heracell VIOS). For high-throughput, biobanking and industrial scale-up, choose a reach-in model from 400 to 820 litres. In addition, stackability plays a role with limited floor space.

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